abstract：:Cellulose degrading enzymes usually have a two-domain structure consisting of a catalytic domain and a non-catalytic carbohydrate-binding module. Although it is well known the importance of those modules in cell wall degrading process, their function is not yet fully understood. Here, we analyze the cellulose-hydrolys...

journal_title：AMB Express

authors： Mello BL,Polikarpov I

更新日期：2014-04-25 00:00:00

abstract：:With fossil fuel sources in limited supply, microalgae show tremendous promise as a carbon neutral source of biofuel. Current microalgae biofuel strategies typically rely on growing high-lipid producing laboratory strains of microalgae in open raceways or closed system photobioreactors. Unfortunately, these microalgae...

journal_title：AMB Express

authors： Eibl JK,Corcoran JD,Senhorinho GN,Zhang K,Hosseini NS,Marsden J,Laamanen CA,Scott JA,Ross GM

更新日期：2014-03-26 00:00:00

abstract：:Most whole cell biocatalysts have some problems with yields and productivities because of various metabolites produced as byproducts and limitations of substrate uptake. We propose a psychrophile-based simple biocatalyst for efficient bio-production using mesophilic enzymes expressed in psychrophilic Shewanella living...

journal_title：AMB Express

authors： Tajima T,Fuki K,Kataoka N,Kudou D,Nakashimada Y,Kato J

更新日期：2013-12-05 00:00:00

abstract：:The potential of Lactobacillus johnsonii NCC 533 to metabolize chlorogenic acids from green coffee extract was investigated. Two enzymes, an esterase and a hydroxycinnamate decarboxylase (HCD), were involved in this biotransformation. The complete hydrolysis of 5-caffeoylquinic acid (5-CQA) into caffeic acid (CA) by L...

journal_title：AMB Express

authors： Bel-Rhlid R,Thapa D,Kraehenbuehl K,Hansen CE,Fischer L

更新日期：2013-05-21 00:00:00

abstract：:Lactobacillus panis strain PM1 is an obligatory heterofermentative and aerotolerant microorganism that also produces 1,3-propanediol from glycerol. This study investigated the metabolic responses of L. panis PM1 to oxidative stress under aerobic conditions. Growth under aerobic culture triggered an early entrance of L...

journal_title：AMB Express

authors： Kang TS,Korber DR,Tanaka T

更新日期：2013-01-31 00:00:00

abstract：: In this study manganese peroxidase (MnP) enzymes from selected white-rot fungi were isolated and compared for potential future recombinant production. White-rot fungi were cultivated in small-scale in liquid media and a simplified process was established for the purification of extracellular enzymes.Five lignin degra...

journal_title：AMB Express

authors： Järvinen J,Taskila S,Isomäki R,Ojamo H

更新日期：2012-11-29 00:00:00

abstract：: The nutrition-versatility of Burkholderia sp. strain USM (JCM 15050) has initiated the studies on the use of this bacterium for polyhydroxyalkanoate (PHA) production. To date, the Burkholderia sp. has been reported to synthesize 3-hydroxybutyrate, 3-hydroxyvalerate and 3-hydroxy-4-methylvalerate monomers. In this stu...

journal_title：AMB Express

authors： Lau NS,Sudesh K

更新日期：2012-08-09 00:00:00

abstract：: To improve the biodegradation of biodegradable plastic (BP) mulch films, 1227 fungal strains were isolated from plant surface (phylloplane) and evaluated for BP-degrading ability. Among them, B47-9 a strain isolated from the leaf surface of barley showed the strongest ability to degrade poly-(butylene succinate-co-bu...

journal_title：AMB Express

authors： Koitabashi M,Noguchi MT,Sameshima-Yamashita Y,Hiradate S,Suzuki K,Yoshida S,Watanabe T,Shinozaki Y,Tsushima S,Kitamoto HK

更新日期：2012-08-02 00:00:00

abstract：: Vinasse, a highly polluting waste of the ethanol industry was utilized for the production of polyhydroxyalkanoate (PHA) by the extremely halophilic archaeon, Haloferax mediterranei in shake-flasks. Following pre-treatment through adsorption on activated carbon, 25%-50% (v/v) pre-treated vinasse was utilized leading t...

journal_title：AMB Express

authors： Bhattacharyya A,Pramanik A,Maji SK,Haldar S,Mukhopadhyay UK,Mukherjee J

更新日期：2012-07-09 00:00:00

abstract：: In this study, we used the yeast carotenogenic producer Pichia pastoris Pp-EBIL strain, which has been metabolically engineered, by heterologously expressing β-carotene-pathway enzymes to produce β-carotene, as a vessel for recombinant astaxanthin expression. For this purpose, we designed new P. pastoris recombinant-...

journal_title：AMB Express

authors： Araya-Garay JM,Ageitos JM,Vallejo JA,Veiga-Crespo P,Sánchez-Pérez A,Villa TG

更新日期：2012-04-25 00:00:00

abstract：: Two genes from Streptomyces albidoflavus, one exochitinase (905-bp) and an endochitinase (1100-bp) were functionally expressed in Escherichia coli in form of a fusion protein with a maltose binding protein (MBP). The goal was to produce and test proteins that inhibit both the coffee berry borer insect Hypothenemus ha...

journal_title：AMB Express

authors： Martínez CP,Echeverri C,Florez JC,Gaitan AL,Góngora CE

更新日期：2012-03-30 00:00:00

abstract：: Biocathode MFCs using microorganisms as catalysts have important advantages in lowering cost and improving sustainability. Electrode materials and microbial synergy determines biocathode MFCs performance. In this study, four materials, granular activated carbon (GAC), granular semicoke (GS), granular graphite (GG) an...

journal_title：AMB Express

authors： Sun Y,Wei J,Liang P,Huang X

更新日期：2012-03-29 00:00:00

abstract：: Type-2 diabetes is mediated by defects in either insulin secretion or insulin action. In an effort to identify extracts that may stimulate glucose uptake, similar to insulin, a high throughput-screening assay for measuring glucose uptake in skeletal muscle cells was established. During the screening studies to discov...

journal_title：AMB Express

authors： Kulkarni-Almeida AA,Brahma MK,Padmanabhan P,Mishra PD,Parab RR,Gaikwad NV,Thakkar CS,Tokdar P,Ranadive PV,Nair AS,Damre AA,Bahirat UA,Deshmukh NJ,Doshi LS,Dixit AV,George SD,Vishwakarma RA,Nemmani KV,Mahajan GB

更新日期：2011-11-21 00:00:00

abstract：: In winemaking, nutrient supplementation is a common practice for optimising fermentation and producing quality wine. Nutritionally suboptimal grape juices are often enriched with nutrients in order to manipulate the production of yeast aroma compounds. Nutrients are also added to active dry yeast (ADY) rehydration me...

journal_title：AMB Express

authors： Winter G,Henschke PA,Higgins VJ,Ugliano M,Curtin CD

更新日期：2011-11-02 00:00:00

abstract：: Effective utilization of photosynthetic microorganisms as potential biocatalysts is favorable for the production of useful biomaterials and the reduction of atmospheric CO2. For example, biocatalytic transformations are used in the synthesis of optically active alcohols. We previously found that ketone reduction in c...

journal_title：AMB Express

authors： Yamanaka R,Nakamura K,Murakami A

更新日期：2011-09-01 00:00:00

abstract：: Hydroquinone-1,2-dioxygenase, an enzyme involved in the degradation of alkylphenols in Sphingomonas sp. strain TTNP3 was purified to apparent homogeneity. The extradiol dioxygenase catalyzed the ring fission of hydroquinone to 4-hydroxymuconic semialdehyde and the degradation of chlorinated and several alkylated hydr...

journal_title：AMB Express

authors： Kolvenbach BA,Lenz M,Benndorf D,Rapp E,Fousek J,Vlcek C,Schäffer A,Gabriel FL,Kohler HP,Corvini PF

更新日期：2011-05-27 00:00:00

abstract：: Biosurfactants are amphiphilic molecules that have both hydrophilic and hydrophobic moieties which partition preferentially at the interfaces such as liquid/liquid, gas/liquid or solid/liquid interfaces. Such characteristics enable emulsifying, foaming, detergency and dispersing properties. Their low toxicity and env...

journal_title：AMB Express

authors： Makkar RS,Cameotra SS,Banat IM

更新日期：2011-03-28 00:00:00